Argonaute and Dicer are essential for communication between Trichoderma atroviride and fungal hosts during mycoparasitism
Artículo
Te invitamos a leer el artículo "Argonaute and Dicer are essential for communication between Trichoderma atroviride and fungal hosts during mycoparasitism" publicado en Microbiology Spectrum, a cargo del profesor investigador Dr. Alfredo Heriberto Herrera Estrella y su equipo de trabajo de la UGA-Langebio.
Autores: Eli Efrain Enriquez-Felix / Camilo Pérez-Salazar, / José Guillermo Rico-Ruiz / Ana Calheiros de Carvalho / Pablo Cruz-Morales / José Manuel Villalobos-Escobedo / Alfredo Herrera-Estrella
- Laboratorio Nacional de Genómica para la Biodiversidad-Unidad de Genómica Avanzada, Cinvestav Campus Guanajuato, Irapuato, 36821, Guanajuato, México
- Novo Nordisk Center for Biosustainability, Technical University of Denmark.
- The LatAmBio Initiative
- Department of Plant and Microbial Biology, University of California, Berkeley, CA, U.S.
- Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, U.S.
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Summary:
JTrichoderma species are known for their mycoparasitic activity against phytopathogenic fungi that cause significant economic losses in agriculture. During mycoparasitism, Trichoderma spp. recognize molecules produced by the host fungus and release secondary metabolites and hydrolytic enzymes to kill and degrade the host’s cell wall. Here, we explored the participation of the Trichoderma atroviride RNAi machinery in the interaction with six phytopathogenic fungi of economic importance. We determined that both Argonaute-3 and Dicer-2 play an essential role during mycoparasitism. Using an RNA-Seq approach, we identified that perception, detox, and cell wall degradation depend on the T. atroviride-RNAi when interacting with Alternaria alternata, Rhizoctonia solani AG2, and R. solani AG5. Furthermore, we constructed a gene co-expression network that provides evidence of two gene modules regulated by RNAi, which play crucial roles in essential processes during mycoparasitism. In addition, based on small RNA-seq, we conclude that siRNAs regulate amino acid and carbon metabolism and communication during the Trichoderma-host interaction. Interestingly, our data suggest that siRNAs might regulate allorecognition (het) and transport genes in a cross-species manner. Thus, these results reveal a fine-tuned regulation in T. atroviride dependent on siRNAs that is essential during the biocontrol of phytopathogenic fungi, showing a greater complexity of this process than previously established.