Backcrossing Modulates the Metabolic Profiles of Anthocyanin-Pigmented ‘Vitamaize’ Lines Derived from Elite Maize Lines
Artículo
Te invitamos a leer el artículo "Backcrossing Modulates the Metabolic Profiles of Anthocyanin-Pigmented ‘Vitamaize’ Lines Derived from Elite Maize Lines" publicado en Plant Foods for Human Nutrition,a cargo del profesor investigador Dr. Robert Winkler y su equipo de trabajo de la UGA-Langebio.
Autores: Héctor Arturo Peniche-Pavía / Tzitziki González-Rodríguez / Axel Tiessen, Silvero García-Lara & Robert Winkler
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Cinvestav Unidad Irapuato
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Centro de Investigación y de Estudios Avanzados del IPN, Unidad de Genómica Avanzada-Langebio
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Tecnológico de Monterrey
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Summary:
Vitamaize lines (VMLs) were created by backcrossing the pigmented aleurone trait into Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) maize lines (CMLs). This study evaluates metabolic differences between the VMLs and their original CMLs. Direct infusion mass spectrometry (DIMS) analyses, carotenoid profiling, total anthocyanins content (TAC) determination, and biochemical evaluation of the quality protein maize (QPM) endosperm trait allowed a comprehensive chemical characterization of the maize lines. DIMS data indicate higher hexoses and trigonelline content for most VMLs; the carotenoid profile revealed a decrease in β-cryptoxanthin to less than half of the original parent content for two VMLs but an augmentation for one VML. The pigmented aleurone VMLs did not inherit the complex QPM endosperm trait of the QPM CMLs. Except for anthocyanin accumulation, no other metabolites were consistently modified across all the backcross-generated maize lines with a pigmented aleurone trait. These findings suggest using genetic or metabolic markers rather than morphological or visual traits for future breeding programs.