DNA repair during regeneration in Ambystoma mexicanum

11 de enero de 2021

Te invitamos a leer: “DNA repair during regeneration in Ambystoma mexicanum” publicado en Developmental Dynamics, colaboración del Departamento de Genética y Biología Molecular. Unidad Zacatenco Cinvestav y el Laboratorio Complejidad Molecular y del Desarrollo de la UGA-Langebio.

Autores: Ulises Omar García-Lepe¹, Alfredo Cruz-Ramírez², Rosa María Bermúdez-Cruz¹.

1. Genetics and Molecular Biology Department, Centro de Investigacion y Estudios Avanzados del IPN, Mexico city, Mexico.
2. Molecular and Developmental Complexity Group, Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.

Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.

Abstract: The remarkable regenerative capabilities of the salamander Ambystoma mexicanum have turned it into one of the principal models to study limb regeneration. During this process, a mass of low differentiated and highly proliferative cells, called blastema, propagates to reestablish the lost tissue in an accelerated way. Such a process implies the replication of a huge genome, 10 times larger than humans, with about 65.6% of repetitive sequences. These features make the axolotl genome inherently difficult to replicate and prone to bear mutations. In this context, the role of DNA repair mechanisms acquires great relevance to maintain genomic stability, especially if we consider the necessity of ensuring the correct replication and integrity of such a large genome in the blastema cells, which are key for tissue regeneration. On the contrary, DNA damage accumulation in these cells may result in senescence, apoptosis and premature differentiation, all of them are mechanisms employed to avoid DNA damage perpetuation but with the potential to affect the limb regeneration process. Here we review and discuss the current knowledge on the implications of DNA damage responses during salamander regeneration.

Keywords: Ambystoma mexicanum, DNA damage, DNA repair, genomic stability, limb regeneration, stemcells