Expression attenuation as a mechanism of robustness against gene duplication
09 de febrero de 2021
Te invitamos a leer: “Expression attenuation as a mechanism of robustness against gene duplication” publicado en PNAS, trabajo del Dr. Alexander De Luna, Profesor investigador del Laboratorio de Sistemas Genéticos de la UGA-Langebio, en colaboración con la Université Laval, Québec y equipo de trabajo.
Autores: Diana Ascenci, Guillaume Diss, Isabelle Gagnon-Arsenault, Alexandre K. Dubé, Alexander DeLuna, and Christian R. Landry.
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Abstract: Gene duplication is ubiquitous and a major driver of phenotypic diversity across the tree of life, but its immediate consequences are not fully understood. Deleterious effects would decrease the probability of retention of duplicates and prevent their contribution to long-term evolution. One possible detrimental effect of duplication is the perturbation of the stoichiometry of protein complexes. Here, we measured the fitness effects of the duplication of 899 essential genes in the budding yeast using high-resolution competition assays. At least 10% of genes caused a fitness disadvantage when duplicated. Intriguingly, the duplication of most protein complex subunits had small to nondetectable effects on fitness, with few exceptions. We selected four complexes with subunits that had an impact on fitness when duplicated and measured the impact of individual gene duplications on their protein–protein interactions. We found that very few duplications affect both fitness and interactions. Furthermore, large complexes such as the 26S proteasome are protected from gene duplication by attenuation of protein abundance. Regulatory mechanisms that maintain the stoichiometric balance of protein complexes may protect from the immediate effects of gene duplication. Our results show that a better understanding of protein regulation and assembly in complexes is required for the refinement of current models of gene duplication.
Keywords: gene duplication, gene expression, protein interaction, dosage balancehypothesis,fitness effects.