Extracellular Self- and Non-Self DNA Involved in Damage Recognition in the Mistletoe Parasitism of Mesquite Trees

Claudia Marina López García, César Alejandro Ávila Hernández, Elizabeth Quintana Rodríguez, Víctor Aguilar Hernández, Nancy Edith Lozoya Pérez, Mariana Atzhiry Rojas Raya, Jorge Molina Torres, Jesús Alfredo Araujo-León, Ligia Brito Argáez, Avel Adolfo González Sánchez, Enrique Ramírez Chávez and Domancar Orona Tamayo

Te invitamos a leer el artículo "Extracellular Self- and Non-Self DNA Involved in Damage Recognition in the Mistletoe Parasitism of Mesquite Trees"  publicado Molecular Sciences, en el que colaboraron   César Alejandro Ávila Hernández, Mariana Atzhiry Rojas Raya,  el Dr. Jorge Molina Torres y Enrique Ramírez Chávez de la Unidad Irapuato.

Autores:

Claudia Marina López García, César Alejandro Ávila Hernández, Elizabeth Quintana Rodríguez, Víctor Aguilar Hernández, Nancy Edith Lozoya Pérez, Mariana Atzhiry Rojas Raya, Jorge Molina Torres, Jesús Alfredo Araujo-León, Ligia Brito Argáez, Avel Adolfo González Sánchez, Enrique Ramírez Chávez and Domancar Orona Tamayo.

Resumen:

Psittacanthus calyculatus parasitizes mesquite trees through a specialized structure called a haustorium, which, in the intrusive process, can cause cellular damage in the host tree and release DAMPs, such as ATP, sugars, RNA, and DNA. These are highly conserved molecules that primarily function as signals that trigger and activate the defense responses. In the present study, we generate extracellular DNA (exDNA) from mesquite (P. laevigata) tree leaves (self-exDNA) and P. calyculatus (non-self exDNA) mistletoe as DAMP sources to examine mesquite trees’ capacity to identify specific self or non-self exDNA. We determined that mesquite trees perceive self- and non-self exDNA with the synthesis of O2•−, H2O2, flavonoids, ROS-enzymes system, MAPKs activation, spatial concentrations of JA, SA, ABA, and CKs, and auxins. Our data indicate that self and non-self exDNA application differs in oxidative burst, JA signaling, MAPK gene expression, and scavenger systems. This is the first study to examine the molecular biochemistry effects in a host tree using exDNA sources derived from a mistletoe.