lncRNAs involved in the Shade AvoidanceSyndrome (SAS) in Arabidopsis thaliana
Artículo
Te invitamos a leer el artículo "lncRNAs involved in the Shade AvoidanceSyndrome (SAS) in Arabidopsis thaliana" publicado en BMC Genomics, a cargo del profesor investigador Dr. Stewart Gillmor y su equipo de trabajo de la UGA.
Autores: Irving Jair García-López / Aarón I. Vélez-Ramírez / C. Stewart Gillmor / Selene L. Fernandez-Valverde
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Laboratorio de Genética y Epigenética de Semillas, Unidad de Genómica Avanzada (UGA). México, Cinvestav, Mexico
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Summary:
Background
Plant long non-coding RNAs (lncRNAs) have important regulatory roles in responses to various biotic and abiotic stresses, including light quality. However, no lncRNAs have been specifically linked to the Shade Avoidance Response (SAS).
Results
To better understand the involvement of lncRNAs in shade avoidance, we examined RNA-seq libraries for lncRNAs with the potential to function in the neighbor proximity phenomenon in Arabidopsis thaliana (A. thaliana). Using transcriptomes generated from seedlings exposed to high and low red/far-red (R/FR) light conditions, we identified 13 lncRNA genes differentially expressed in cotyledons and 138 in hypocotyls. To infer possible functions for these lncRNAs, we used a ‘guilt-by-association’ approach to identify genes co-expressed with lncRNAs in a weighted gene co-expression network. Of 34 co-expression modules, 10 showed biological functions related to differential growth. We identified three potential lncRNAs co-regulated with genes related to SAS. T-DNA insertions in two of these lncRNAs were correlated with morphological differences in seedling responses to increased FR light, supporting our strategy for computational identification of lncRNAs involved in SAS.
Conclusions
Using a computational approach, we identified multiple lncRNAs in Arabidopsis involved in SAS. T-DNA insertions caused altered phenotypes under low R/FR light, suggesting functional roles in shade avoidance. Further experiments are needed to determine the specific mechanisms of these lncRNAs in SAS.