RNA Isolation Method in Marginal Crops with High Agronomic Potential

Kena Casarrubias Castillo, John Paul Délano Frier y Paola Andrea Palmeros Suárez

Te invitamos a leer el capítulo "RNA Isolation Method in Marginal Crops with High Agronomic Potential" publicado en el libro "Plant Functional Genomics", en el que colaboró el Dr.John Paul Délano Frier de Cinvestav Irapuato.

Autores:

Kena Casarrubias Castillo, John Paul Délano Frier y Paola Andrea Palmeros Suárez

Resumen:

Ribonucleic Acid (RNA) isolation is a basic technique in the field of molecular biology. The purpose of RNA isolation is to acquire pure and complete RNA that can be used to evaluate gene expression. Many methods can be used to perform RNA isolation, all of them based on the chemical properties of nucleic acids. However, some of them do not achieve high RNA yields and purity levels when used in a number of marginally studied crops of agronomic importance, such as grain and vegetable amaranth plants. In the method described here, the use of guanidinium thiocyanate and two additional precipitation steps with different reagents designed to obtain high yields and RNA purity levels from diverse plant species employed for plant functional genomics studies is described.