Mónica Lamas - Müller Glia to Müller Glia Extracellular Vesicle-Dependent Signaling Induces Multipotency Genes Nestin and lin28 Expression in Response to N-methyl-D-aspartate (NMDA) Exposure
4 de Junio de 2023
Invitamos a leer el artículo: “Müller Glia to Müller Glia ExtracellularVesicle-Dependent Signaling InducesMultipotency Genes Nestin and lin28Expression in Response toN-methyl-D-aspartate (NMDA) Exposure”, en la que colaboró la Doctora Monica Lamas, Investigadora de Cinvestav Sede Sur
Autores: Ana Karen Carapia, Erick J. Martinez Colin,Deisy Segura Villalobos, Rebeca Yael Victoria Chavez,Ivonne Lezama, Eduardo Martinez Martinez y Mónica Lamas
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Abstract: A strict localization into the neural retina and the capacity to survive many pathogenic conditions are unique characteristics ofMüller glia. Retinal damage induces cellular and molecular changes that place Müller cells as focal points to either protect orfurther impair neuronal function. This work evaluated the involvement of extracellular vesicles (EVs) in the transduction ofhigh N-methyl-D-aspartate (NMDA) concentration signal between Müller glia-to-Müller glia as part of several factors and sig-nal transduction mechanisms dictating either a gliotic (damaging) or regenerative process. These EVs were obtained, charac-terized, and quantified from control and NMDA-treated Müller cells using murine Müller cell primary cultures to evaluate thefunctional outcome of these preparations. The obtained results support the existence of different subpopulations of EVs thatmay induce a differential response. These EVs did not affect the viability, migration, and wound healing capacity of culturedMüller cells. However, the EVs obtained from NMDA-treated Müller cultures, but not from untreated cells, increasedactin polymerization and induced the expression of the multipotency genes nestin and lin28. We propose that, upon a lesionparadigm, Müller-derived EVs may induce the spread of molecular changes in neighboring cells resulting in the formation of thecharacteristic glial retinal scar in mammalians or else trigger a neurogenic process in regenerative species
Keywords: extracellular vesicles, dedifferentiation, Müller glia, retina, stem cell